Detailed Information |
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| Standard Gene Set Name | GREGORY_ABH1-MUTATION_UP |
| Species | Arabidopsis thaliana |
| Brief Description | Up-regulated upon ABH1 mutation (abh1-1) (TableS1 PubmedID:18486559) |
| Full Description/Abstract | MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are abundant endogenous small RNAs (smRNAs) that control transcript expression through posttranscriptional gene silencing. Here, we show that concomitant loss of XRN4/EIN5, a 5-3 exoribonuclease, and ABH1/CBP80, a subunit of the mRNA cap binding complex, results in Arabidopsis plants manifesting myriad developmental defects. We find that ABH1/CBP80 is necessary to obtain proper mature miRNA levels, which suggests this protein affects the miRNA-mediated RNA silencing pathway. Additionally, we show that XRN4/EIN5 affects the levels of a smRNA class that is processed from both sense and antisense strands of 130 endogenous transcripts that apparently are converted to double-stranded RNA (dsRNA) and subsequently processed. We find that the parent transcripts of these smRNAs accumulate in an uncapped form upon loss of XRN4/EIN5, which suggests that uncapped endogenous transcripts can become smRNA biogenesis substrates. Overall, our results reveal unexpected connections between RNA metabolism and silencing pathways |
| External Pathway ID/Pubmed ID | 18486559 |
| Source | Literature |
| Contributor/Author | Liming Lai and Xijin Ge |
| Organization of contributer | South Dakota State University |
| External URL | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WW3-4SJY910-1-3&_cdi=7119&_user=10&_coverDate=06%2F10%2F2008&_sk=%23TOC%237119%232008%23999859993%23691870%23FLA%23display%23Volume_14,_Issue_6,_Pages_811-970_(10_June_2008)%23tagged%23Volume%23first%3D14%23Issue%23first%3D6%23Date%23(10_June_2008)%23&view=c&_gw=y&wchp=dGLbVlb-zSkWA&md5=3a16378ee10f23972941d74810e8fe83&ie=/sdarticle.pdf |