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| Standard Gene Set Name | GERMAN_MIR169N |
| Species | Arabidopsis thaliana |
| Brief Description | Putative targets of mir169N by German et al. Global identification of microRNA¨Ctarget RNA pairs by parallel analysis of RNA ends. Nature Biotechnology 26:941-946. |
| Full Description/Abstract | MicroRNAs (miRNAs) are important regulatory molecules in most eukaryotes and identification of their target mRNAs is essential for their functional analysis. Whereas conventional methods rely on computational prediction and subsequent experimental validation of target RNAs, we directly sequenced >28,000,000 signatures from the 5' ends of polyadenylated products of miRNA-mediated mRNA decay, isolated from inflorescence tissue of Arabidopsis thaliana, to discover novel miRNA¨Ctarget RNA pairs. Within the set of 27,000 transcripts included in the 8,000,000 nonredundant signatures, several previously predicted but nonvalidated targets of miRNAs were found. Like validated targets, most showed a single abundant signature at the miRNA cleavage site, particularly in libraries from a mutant deficient in the 5'-to-3' exonuclease AtXRN4. Although miRNAs in Arabidopsis have been extensively investigated, working in reverse from the cleaved targets resulted in the identification and validation of novel miRNAs. This versatile approach will affect the study of other aspects of RNA processing beyond miRNA¨Ctarget RNA pairs. |
| External Pathway ID/Pubmed ID | mir169N |
| Source | miRNA |
| Contributor/Author | Liming Lai and Xijin Ge |
| Organization of contributer | South Dakota State University |
| External URL | http://mpss.udel.edu/at_pare/ |